Hydration of an Oriental Spruce (<I>Picea orientalis</I>) Pollen Grain
C. John Runions University of Victoria Centre for Forest Biology Victoria British Columbia V8W 3N5 Canada
Picea, Pinaceae, pollen, pollination, sacci, C. John Runions, Kim H. Rensing, Tokushiro Takaso, John N. Owens
BSA Plant Images Online -
Time series during hydration of an oriental spruce (Picea orientalis) pollen grain. Rhodamine B stains the exine (red) while fluorescein diacetate crosses plasmalemmae and indicates esterase activity in the living cells (green). Swelling of the tube cell reduces the volume of the saccate air space and results in a loss of pollen buoyancy. Confocal extended depth of focus sections taken at (top to bottom) 1, 8, and 15 min after start of hydration.